Pseudomonas aeruginosa is a major opportunistic pathogen, causing a wide range of acute and chronic infections. ?-lactam antibiotics including penicillins, carbapenems, monobactams, and cephalosporins play a key role in the treatment of P. aeruginosa infections. However, a significant number of isolates of these bacteria are resistant to ?-lactams, complicating treatment of infections and leading to worse outcomes for patients. Resistance to ?-lactams is multifactorial and can involve changes to a key target protein, penicillin-binding protein 3, that is essential for cell division; reduced uptake or increased efflux of ?-lactams; degradation of ?-lactam antibiotics by increased expression or altered substrate specificity of an AmpC ?-lactamase, or by the acquisition of ?-lactamases through horizontal gene transfer; and changes to biofilm formation and metabolism. The table 1 shows that all the soil samples gotten from the different faculties in unizik have Pseudomonas spp. The ?-lactam antibiotics test carried out on the Pseudomonas spp gotten from different soil samples as shown in table 2,3 and 4 shows that in soil sample B is resistant to ampicilin in 250mg/L, 125mg/L and also Resistant in 125mg/L of ciprofloxacin. While in table 4, soil sample B, C, and E are Resistant in 125mg/L of Chloramphenicol. The total heterotrophic bacteria count of P. aeruginosa on nutrient agar in table 5 shows that sample C has the highest number of P. aeruginosa (9.70 x 104cfu/ml) while sample B is the lowest (1.88 x 105 cfu/ml).