Reviewer
| Name: | Dr. Sunil Bhausaheb Kokane? |
| Designation: | Assistant Professor |
| Department: | Molecular Biology and Biotechnology |
| Research Area: | Biotechnology, Molecular Biology, Plant Virology, Pathology |
| Organization: | Shuats Allahabd |
| Contact: | Contact Reviewer |
| About: | The major thrust of my work is on molecular detection, characterization, and management of important virus / virus-like pathogens infecting citrus in India viz. Citrus tristeza virus, Candidatus Liberibacter spp. (Citrus greening), Indian citrus ringspot virus, Citrus yellow mosaic virus, and Citrus phytoplasma. I am well-versed in developing molecular diagnostic tools, biological indexing, disease transmission studies, and taking care of polyhouse activities like raising indicator plant seedlings, maintaining virus cultures etc. I know different molecular biology techniques like PCR, RT-PCR, Real Time PCR, Electrophoresis, PAGE, LAMP, ELISA, RFLP analysis, Recombinase polymerase amplification (RPA) based LFA diagnosis, protein expression and purification. I am also handling different bioinformatics software’s viz. MEGA, Bioedit, Primer designing software’s, NCBI, BLAST etc. Our research findings have been published in reputed international journals like Scientific Reports (Nature), Phytopathology (The American Phytopathological Society), FEBS journal (Wiley), Metallomics (The Royal Society of Chemistry), PLoS One, Plant Disease (The American Phytopathological Society), Molecular and Cellular Probes (Elsevier), Journal of Virological methods (Elsevier), 3 Biotech (Springer), Biologia Plantarum, Journal of Plant Biochemistry & Biotechnology (Springer) etc. While working at ICAR-CCRI, Nagpur, I also had an opportunity to attend training programs at other research organizations like ABGENEX, Bhubaneswar (two weeks), National Center for Microbial Resource, NCCS, Pune (one week), and ICAR-National Research Centre for Banana, Tiruchirapalli, Tamil nadu (two weeks). I also worked as a resource person to provide training on “PCR diagnosis of virus and virus-like pathogen including Citrus greening (HLB) and advanced techniques of citrus nursery plant propagation system†(two weeks) to agriculture officer of the royal government of Bhutan and to the staff of College o |
| Status: |
| Author: | Ghosh, D.K., Kokane, S.B., Kokane, A., Warghane, A., Motghare, M., Bhose, S., Sharma, A.K. Reddy, M.K. |
| Title: | Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of |
| Publication Year: ISSN: Volume: Issue: |
2018 1932-6203 13 e0208530 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Ghosh, D.K., Kokane, S.B., Kumar, P., Ozcan, A., Warghane, A., Motghare, M., Santra, S., Sharma, A.K. |
| Title: | Antimicrobial Nano-Zinc Oxide-2S Albumin protein formulation significantly inhibits growth of Candidatus Liberibacter asiaticus in planta |
| Publication Year: ISSN: Volume: Issue: |
2018 1932-6203 13 e0204702 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Kokane, A., Kokane, S.B., Warghane, A. J., Gubyad, M. G., Sharma, A. K., Reddy M. K., Ghosh, D. K. |
| Title: | A Rapid and Sensitive Reverse Transcription–Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Detection of Indian Citrus Ringspot Virus |
| Publication Year: ISSN: Volume: Issue: |
2021 0191-2917 105 1346-1355 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Ghosh, D.K., Kokane, S.B., Gowda, S. |
| Title: | Development of a reverse transcription recombinase polymerase based isothermal amplification coupled with lateral flow immunochromatographic assay (CTV-RT-RPA-LFICA) for rapid detection of Citrus tristeza virus |
| Publication Year: ISSN: Volume: Issue: |
2020 2045-2322 10 10: 20593 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Kokane, S.B., Misra, P., Kokane, A.D., Gubyad, M.G., Warghane, A.J., Surwase, D., Reddy, M.K., Ghosh, D.K. |
| Title: | Development of a real-time RT-PCR method for the detection of Citrus tristeza virus (CTV) and its implication in studying virus distribution in planta |
| Publication Year: ISSN: Volume: Issue: |
2021 2190-5738 11 10 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, NAAS, ABCD-Index, |
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| Author: | Kokane, A.D., Lawrence, K., Kokane, S.B., Gubyad, M.G., Misra, P., Reddy, M.K., Ghosh, D.K. |
| Title: | Development of a SYBR Green-based RT-qPCR assay for the detection of Indian citrus ringspot virus |
| Publication Year: ISSN: Volume: Issue: |
2021 2190-5738 11 7 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Ghosh, D.K., Kokane, A., Kokane, S.B., Tenzin, J., Gubyad, M.G., Wangdi, P., Murkute, A.A. Sharma, A.K, Gowda, S. |
| Title: | Detection and molecular characterization of ‘Candidatus Liberibacter asiaticus’ and Citrus tristeza virus associated with citrus decline in Bhutan |
| Publication Year: ISSN: Volume: Issue: |
2021 0031-949X 111, No. 5 PHYTO-07-20-0266-R |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Kokane, S.B., Kokane, A.D., Misra, P., Warghane, A., Kumar, P., Gubyad, M.G., Sharma, A.K., Biswas, K.K., Ghosh D.K. |
| Title: | In-silico characterization and RNA-binding protein based polyclonal antibodies production for detection of Citrus tristeza virus |
| Publication Year: ISSN: Volume: Issue: |
2020 0890-8508 54 101654 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Sunil B Kokane, Sumit Bhose, Amol Kokane, Mrugendra Gubyad, Dilip Kumar Ghosh |
| Title: | Molecular detection, identification, and sequence analysis of Candidatus Liberibacter asiaticus associated with Huanglongbing disease of citrus in North India |
| Publication Year: ISSN: Volume: Issue: |
2020 2190-5738 10 8 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Pranav Kumar, Vikram Dalal, Nidhi Sharma, Sunil Kokane, Dilip Kumar Ghosh, Pravindra Kumar, Ashwani Kumar Sharma |
| Title: | Characterization of the heavy metal binding properties of periplasmic metal uptake protein CLas-ZnuA2+ |
| Publication Year: ISSN: Volume: Issue: |
2020 1756-5901 12 2 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Warghane, A., Kokane, D., Kokane, S.B., Motghare, M., Surwase, D., Chodhury, S., Biswas, K., Ghosh, D.K |
| Title: | Molecular detection and coat protein gene based characterization of Citrus tristeza virus prevalent in Sikkim state of India |
| Publication Year: ISSN: Volume: Issue: |
2020 0367-973X 73 1 |
| Indexing: | SCOPUS, ABCD-Index, |
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| Author: | Pranav Kumar, Pooja Kesari, Sunil Kokane, Dilip Kumar Ghosh, Pravindra Kumar, Ashwani Kumar Sharma |
| Title: | Crystal Structures of a putative periplasmic cystineâ€binding protein from Candidatus Liberibacter asiaticus: Insights into an adapted mechanism of ligand binding |
| Publication Year: ISSN: Volume: Issue: |
2019 1742-464X 286 17 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Motghare, M., Kokane, A., Warghane, A., Kokane, S.B., Sharma, A.K., Ghosh, D.K. |
| Title: | Quantitative distribution of Citrus yellow mosaic badnavirus in sweet orange (Citrus sinensis) and its implication in developing disease diagnostics |
| Publication Year: ISSN: Volume: Issue: |
2018 0166-0934 259 1 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Kokane, S.B., Pathak, R.K, Singh, M, Kumar, A. |
| Title: | The role of tripartite interaction of calcium sensors and transporters in the accumulation of calcium in finger millet grain |
| Publication Year: ISSN: Volume: Issue: |
2018 0006-3134 62 2 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Chavhan, R.L., Verma, M., Hinge, V.R., Kadam, U.S., Kokane S.B. |
| Title: | Sequence analysis of coat protein and molecular profiling of sunflower necrosis virus (SNV) strains from Indian subcontinent |
| Publication Year: ISSN: Volume: Issue: |
2018 0971-7811 27 1 |
| Indexing: | SCOPUS, SCI-E, UGC-CARE, ABCD-Index, |
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| Author: | Ghosh, D.K., Motghare, M., Kokane, A., Kokane, S.B., Warghane, A., Bhose, S., Surwase, D., Ladaniya, M.S. |
| Title: | First report of a Candidatus Phytoplasma cynodontis related strain (group 16SrXIV) associated with Huanglongbing disease on Citrus grandis in India |
| Publication Year: ISSN: Volume: Issue: |
2019 1833-928X 14 1 |
| Indexing: | SCOPUS, ESCI, UGC-CARE, ABCD-Index, |
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