Silver nanoparticles were combined with Ocimum Tenuiflorum L. green and purple (Krishna Tulasi) assortments using a single advance that was eco-friendly. Ultraviolet-Visible spectrophotometer, Fourier transform Infra-red, X-Ray Diffraction, Scanning Electron Microscope (SEM), and Energy Dispersive X-ray spectroscope was used to describe the particle nature of integrated AgNps (EDX). A variety of modifications confirmed the nanoparticle arrangement and UV-Visible spectroscopy was used to quantify the Surface Plasmon retention band. In the FTIR, the presence of the aromatic rings C-H vibration is comparable to the presence of flavonoids and carbonyl gathering in the C-O stretch vibration. The inserted silver nanoparticles can clearly be seen under an SEM microscope, and the particles are estimated to be between l and 100 nm in size. The Energy Dispersive X-ray spectra demonstrate the nanomaterial’s quality. Union is traditional and viewed as proficient as far as response time as well as practicality. The combined nanoparticles were exposed to Anti-microbial and Anti proliferative investigations by in-vitro strategies and showed a portion of the subordinate movement against specific microbes and cancer cells.