SERS detection platform based on a nucleic acid aptamer-functionalized Au nano-dodecahedron array for efficient simultaneous testing of colorectal cancer-associated microRNAs

Shuofeng Zhang; Fengsong Chen; Yanqing Zhang; Yemin Xu; Lu Wang; Xiya Wang; Long Jia; Yong Chen; Yongcheng Xu; Zhengrong Zhang; and Bin Deng

SERS detection platform based on a nucleic acid aptamer-functionalized Au nano-dodecahedron array for efficient simultaneous testing of colorectal cancer-associated microRNAs

Author : Shuofeng Zhang, Fengsong Chen, Yanqing Zhang, Yemin Xu, Lu Wang, Xiya Wang, Long Jia, Yong Chen, Yongcheng Xu, Zhengrong Zhang, and Bin Deng Journa Name: BIOMEDICAL OPTICS EXPRESS Country : USA Volume: 15 issue: 5 Year: 2024 Views : 429

Abstract:

A surface-enhanced Raman scattering (SERS) detection platform was constructed based on Au nano-dodecahedrons (AuNDs) functionalized with nucleic acid aptamer-specific binding and self-assembly techniques. SERS labels were prepared by modifying Raman signaling molecules and complementary aptamer chains and were bound on the aptamer-functionalized AuNDs array. Using this protocol, the limits of detection (LODs) of miR-21 and miR-18a in the serum were 6.8 pM and 7.6 pM, respectively, and the detection time was 5 min. Additionally, miR-21 and miR-18a were detected in the serum of a mouse model of colorectal cancer. The results of this protocol were consistent with quantitative real-time polymerase chain reaction (qRT-PCR). This method provides an efficient and rapid method for the simultaneous testing of miRNAs, which has great potential clinical value for the early detection of colorectal cancer (CRC).