Robust autofocus method based on patterned active illumination and image cross-correlation analysis

Caiwei Li; Kehan Liu; Xiaoguang Guo; Yinghao Xiao; Yingjun Zhang; and Zhen-Li Huang

Robust autofocus method based on patterned active illumination and image cross-correlation analysis

Author : Caiwei Li, Kehan Liu, Xiaoguang Guo, Yinghao Xiao, Yingjun Zhang, and Zhen-Li Huang Journa Name: BIOMEDICAL OPTICS EXPRESS Country : USA Volume: 15 issue: 4 Year: 2024 Views : 403

Abstract:

For the effectiveness of a computer-aided diagnosis system, the quality of whole-slide image (WSI) is the foundation, and a useful autofocus method is an important part of ensuring the quality of WSI. The existing autofocus methods need to balance focusing speed and focusing accuracy, and need to be optimized separately for different samples or scenes. In this paper, a robust autofocus method based on fiber bundle illumination and image normalization analysis is proposed. For various application scenes, it meets the requirements of autofocusing through active illumination, such as bright field imaging and fluorescence imaging. For different structures on samples, it ensures the autofocusing accuracy through image analysis. The experimental results imply that the autofocusing method in this paper can effectively track the change of the distance from the sample to the focal plane and significantly improve the WSI quality.