Isolation and Analysis of Cellulase-Producing Bacteria from Soil Samples

Ankit Kumar; Vipin Kumar Saini; Vikas Kumar; Disha Sharma; Saba Rana; Shalini Mishra

Isolation and Analysis of Cellulase-Producing Bacteria from Soil Samples

Author : Ankit Kumar, Vipin Kumar Saini, Vikas Kumar, Disha Sharma, Saba Rana, Shalini Mishra Journa Name: INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH AND ENGINEERING TRENDS Country : India Volume: 9 issue: 6 Year: 2023 Views : 528

Abstract:

The aim of this study is to reveal the ability of various isolates obtained from soil to produce cellulase. Cellulose is degraded in soils by cellulolytic microorganism such as fungi and bacteria. Soil samples were collected from Shri Ram College nursery. A total of 10 species were isolated from soil. The two isolates were showed better results on cellulolytic activity using Congo red on Carboxy methyl cellulose (CMC) agar plates. A Gram stain test was carried out to identify the two isolates as Gram-positive rods. Morphological and biochemical analysis on the basis of standard indicated that they all associated mainly with members of the Bacillus sp.