In vivo measurement of the biomechanical properties of human skin with motion-corrected Brillouin microscopy

Maria N. Romodina; Asha Parmar; and Kanwarpal Singh

In vivo measurement of the biomechanical properties of human skin with motion-corrected Brillouin microscopy

Author : Maria N. Romodina, Asha Parmar, and Kanwarpal Singh Journa Name: BIOMEDICAL OPTICS EXPRESS Country : USA Volume: 15 issue: 3 Year: 2024 Views : 482

Abstract:

Biomechanical testing of human skin in vivo is important to study the aging process and pathological conditions such as skin cancer. Brillouin microscopy allows the all-optical, non-contact visualization of the mechanical properties of cells and tissues over space. Here, we use the combination of Brillouin microscopy and optical coherence tomography for motion-corrected, depth-resolved biomechanical testing of human skin in vivo. We obtained two peaks in the Brillouin spectra for the epidermis, the first at 7?GHz and the second near 9-10?GHz. The experimentally measured Brillouin frequency shift of the dermis is lower compared to the epidermis and is 6.8?GHz, indicating the lower stiffness of the dermis.