User-friendly oblique plane microscopy on a fully functional commercially available microscope base
Author :
George Sirinakis, Edward S. Allgeyer, Dmitry Nashchekin, and Daniel St. JohnstonJourna Name:
BIOMEDICAL OPTICS EXPRESS Country :
USAVolume:
15 issue:4 Year:2024 Views : 200
Abstract:
In this work we present an oblique plane microscope designed to work seamlessly with a commercially available microscope base. To support all the functionality offered by the microscope base, where the position of the objective lens is not fixed, we adopted a two-mirror scanning geometry that can compensate for changes to the position of the objective lens during routine microscope operation. We showed that within a?±?1 mm displacement range of the 100X, 1.35?NA objective lens away from its designed position, the PSF size increased by <3% and <11% in the lateral and axial dimensions, respectively, while the error in magnification was <0.5% within volumes extending?±?10 µm about the focal plane. Compared to the more traditional scan-lens/galvo-mirror combination, the two-mirror scanning geometry offers higher light efficiency and a more compact footprint, which could be beneficial to all OPM designs regardless of the use of a commercial base or not.
APA:George Sirinakis, Edward S. Allgeyer, Dmitry Nashchekin, and Daniel St. Johnston. (Volume-15, Issue-4 -(Year-2024)). User-friendly oblique plane microscopy on a fully functional commercially available microscope base. Retrieved from https://opg.optica.org/viewmedia.cfm?uri=boe-15-4-2358&seq=0
Chicago:George Sirinakis, Edward S. Allgeyer, Dmitry Nashchekin, and Daniel St. Johnston. "User-friendly oblique plane microscopy on a fully functional commercially available microscope base" Example, Volume-15-issue-4-Year-2024-2156-7085. https://opg.optica.org/viewmedia.cfm?uri=boe-15-4-2358&seq=0.