Single source CARS-based multimodal microscopy system for biological tissue imaging [Invited]

Mingyu Sheng; Yuan Zhao; Zhenguo Wu; Jianhua Zhao; Harvey Lui; Sunil Kalia; and Haishan Zeng

Single source CARS-based multimodal microscopy system for biological tissue imaging [Invited]

Author : Mingyu Sheng, Yuan Zhao, Zhenguo Wu, Jianhua Zhao, Harvey Lui, Sunil Kalia, and Haishan Zeng Journa Name: BIOMEDICAL OPTICS EXPRESS Country : USA Volume: 15 issue: 1 Year: 2024 Views : 456

Abstract:

A coherent anti-Stokes Raman scattering (CARS)-based multimodality microscopy system was developed using a single Ti:sapphire femtosecond laser source for biological imaging. It provides three complementary and co-registered imaging modalities: CARS, MPM (multiphoton microscopy), and RCM (reflectance confocal microscopy). The imaging speed is about 1 frame-per-second (fps) with a digital resolution of 1024?×?1024 pixels. This microscopy system can provide clear 2-dimensional and 3-dimensional images of ex-vivo biological tissue samples. Its spectral selection initiates vibrational excitation in lipid cells (approximately 2850?cm-1) using two filters on the pump and Stokes beam paths. The excitation can be tuned over a wide spectral range with adjustable spectral filters. The imaging capability of this CARS-based multimodal microscopy system was demonstrated using porcine fat, murine skin, and murine liver tissue samples.