Rhodamine-based fluorescent probe for dynamic STED imaging of mitochondria

Xinwei Gao; Songtao Cai; Luwei Wang; Yong Guo; Liwei Liu; Xiaoyu Weng; Kun Huang; Wei Yan; and Junle Qu

Rhodamine-based fluorescent probe for dynamic STED imaging of mitochondria

Author : Xinwei Gao, Songtao Cai, Luwei Wang, Yong Guo, Liwei Liu, Xiaoyu Weng, Kun Huang, Wei Yan, and Junle Qu Journa Name: BIOMEDICAL OPTICS EXPRESS Country : USA Volume: 15 issue: 3 Year: 2024 Views : 494

Abstract:

Stimulated emission depletion (STED) microscopy holds tremendous potential and practical implications in the field of biomedicine. However, the weak anti-bleaching performance remains a major challenge limiting the application of STED fluorescent probes. Meanwhile, the main excitation wavelengths of most reported STED fluorescent probes were below 500?nm or above 600?nm, and few of them were between 500-600?nm. Herein, we developed a new tetraphenyl ethylene-functionalized rhodamine dye (TPERh) for mitochondrial dynamic cristae imaging that was rhodamine-based with an excitation wavelength of 560?nm. The TPERh probe exhibits excellent anti-bleaching properties and low saturating stimulated radiation power in mitochondrial STED super-resolution imaging. Given these outstanding properties, the TPERh probe was used to measure mitochondrial deformation, which has positive implications for the study of mitochondria-related diseases.