Multidrug resistance among organisms causing Urinary Tract Infections (UTIs) is a major public health problem, threatening the effective treatment of UTIs. This study investigated the multi-drug resistance UTIs among male and female students of Nnmadi Azikiwe University, twenty mid-stream urine samples were collected from 10 male and 10 female students from the Department of Applied Microbiology and Brewing. The samples were cultured on different media and identified through the morphological and biochemical characteristics of the isolates. The antimicrobial susceptibility patterns were determined using Kirby-Bauer disc diffusion technique. The results revealed a high incidence of urinary tract infections (UTIs), with 100% of females and 90% of males showing infection. Morphological and biochemical analyses identified forty isolates, with Proteus spp., Klebsiella spp., and Enterobacter spp. being predominant among others. Susceptibility tests for Gram-negative isolates indicated varying responses to antibiotics, with Proteus spp. showing sensitivity to gentamycin, intermediate susceptibility to ofloxacin, and resistance to ciprofloxacin. Klebsiella spp. displayed intermediate susceptibility to ofloxacin and resistance to other antibiotics. E. coli exhibited intermediate susceptibility to ofloxacin and ciprofloxacin, while Enterobacter spp. was resistant to all tested antibiotics. Among Gram-positive isolates, Enterococcus spp. showed susceptibility to all antibiotics except erythromycin and ciprofloxacin, while Staphylococcus was solely sensitive to levofloxacin. Antibiotic resistance poses a significant challenge for both individuals and healthcare professionals. Therefore, there is an urgent need to identify and prevent the proliferation of antimicrobial resistance among uropathogens in community settings.