The evolving dynamics of the modern workplace have brought increased scholarly attention to generational differences, particularly between Millennials and Generation Z. This study aims to provide a comprehensive theoretical and humanistic analysis of work-life balance and workplace behaviour across these two influential cohorts. Millennials, typically defined as individuals born between 1981 and 1996, and Generation Z, born from 1997 onwards, represent a significant portion of the global workforce. Their distinct socio-economic upbringing, technological exposure, and value systems have shaped divergent expectations from professional environments. This research investigates how these generational traits influence attitudes toward work-life balance, organizational commitment, communication styles, and behavioural patterns within the workplace. The evolving workplace has witnessed a significant shift with the increasing participation of Millennials and Generation Z. This study explores generational differences in work-life balance, workplace behavior, and career expectations through a theoretical and humanistic lens. Unlike traditional studies that rely heavily on quantitative data, this research emphasizes psychological needs, emotional perspectives, and socio-economic influences. The findings suggest that Millennials prioritize career growth and work-life integration, whereas Generation Z emphasizes flexibility, mental well-being, and boundary management. The study highlights the need for organizations to adopt empathetic, flexible, and human-centered HR practices to effectively manage a multigenerational workforce.