High contrast breast cancer biomarker semi-quantification and immunohistochemistry imaging using upconverting nanoparticles

Sanathana Konugolu Venkata Sekar; Hui Ma; Katarzyna Komolibus; Gokhan Dumlupinar; Matthias J. Mickert; Krzysztof Krawczyk; and Stefan Andersson-Engels

High contrast breast cancer biomarker semi-quantification and immunohistochemistry imaging using upconverting nanoparticles

Author : Sanathana Konugolu Venkata Sekar, Hui Ma, Katarzyna Komolibus, Gokhan Dumlupinar, Matthias J. Mickert, Krzysztof Krawczyk, and Stefan Andersson-Engels Journa Name: BIOMEDICAL OPTICS EXPRESS Country : USA Volume: 15 issue: 2 Year: 2024 Views : 199

Abstract:

Breast cancer is the second leading cause of cancer death in women. Current clinical treatment stratification practices open up an avenue for significant improvements, potentially through advancements in immunohistochemistry (IHC) assessments of biopsies. We report a high contrast upconverting nanoparticles (UCNP) labeling to distinguish different levels of human epidermal growth factor receptor 2 (HER2) in HER2 control pellet arrays (CPAs) and HER2-positive breast cancer tissue. A simple Fourier transform algorithm trained on CPAs was sufficient to provide a semi-quantitative HER2 assessment tool for breast cancer tissues. The UCNP labeling had a signal-to-background ratio of 40 compared to the negative control.