Fast, multicolour optical sectioning over extended fields of view with patterned illumination and machine learning

Edward N. Ward; Rebecca M. McClelland; Jacob R. Lamb; Roger Rubio-Sánchez; Charles N. Christensen; Bismoy Mazumder; Sofia Kapsiani; Luca Mascheroni; Lorenzo Di Michele; Gabriele S. Kaminski Schierle

Fast, multicolour optical sectioning over extended fields of view with patterned illumination and machine learning

Author : Edward N. Ward, Rebecca M. McClelland, Jacob R. Lamb, Roger Rubio-Sánchez, Charles N. Christensen, Bismoy Mazumder, Sofia Kapsiani, Luca Mascheroni, Lorenzo Di Michele, Gabriele S. Kaminski Schierle, Journa Name: BIOMEDICAL OPTICS EXPRESS Country : USA Volume: 15 issue: 2 Year: 2024 Views : 323

Abstract:

Structured illumination can reject out-of-focus signal from a sample, enabling high-speed and high-contrast imaging over large areas with widefield detection optics. However, this optical sectioning technique is currently limited by image reconstruction artefacts and poor performance at low signal-to-noise ratios. We combine multicolour interferometric pattern generation with machine learning to achieve high-contrast, real-time reconstruction of image data that is robust to background noise and sample motion. We validate the method in silico and demonstrate imaging of diverse specimens, from fixed and live biological samples to synthetic biosystems, reconstructing data live at 11 Hz across a 44 × 44?m2 field of view, and demonstrate image acquisition speeds exceeding 154 Hz.