Combined ion beam irradiation platform and 3D fluorescence microscope for cellular cancer research

Andrew D. Harken; Naresh T. Deoli; Citlali Perez Campos; Brian Ponnaiya; Guy Garty; Grace S. Lee; Malte J. Casper; Shikhar Dhingra; Wenze Li; Gary W. Johnson; Sally A. Amundson; Peter W. Grabham; Eliz

Combined ion beam irradiation platform and 3D fluorescence microscope for cellular cancer research

Author : Andrew D. Harken, Naresh T. Deoli, Citlali Perez Campos, Brian Ponnaiya, Guy Garty, Grace S. Lee, Malte J. Casper, Shikhar Dhingra, Wenze Li, Gary W. Johnson, Sally A. Amundson, Peter W. Grabham, Eliz Journa Name: BIOMEDICAL OPTICS EXPRESS Country : USA Volume: 15 issue: 4 Year: 2024 Views : 208

Abstract:

To improve particle radiotherapy, we need a better understanding of the biology of radiation effects, particularly in heavy ion radiation therapy, where global responses are observed despite energy deposition in only a subset of cells. Here, we integrated a high-speed swept confocally-aligned planar excitation (SCAPE) microscope into a focused ion beam irradiation platform to allow real-time 3D structural and functional imaging of living biological samples during and after irradiation. We demonstrate dynamic imaging of the acute effects of irradiation on 3D cultures of U87 human glioblastoma cells, revealing characteristic changes in cellular movement and intracellular calcium signaling following ionizing irradiation.