Abstract Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060?nm, 1250?nm) for two-photon excitation of red-shifted fluorophores. These custom excitation sources are both simple to construct and cost-efficient in comparison to similar custom and commercial alternatives. Furthermore, they operate at a repetition rate (80?MHz) that allows fast image acquisition using resonant scanners. With our system we demonstrate compatibility with fast resonant scanning, the ability to acquire neuronal images, and the capability to image vasculature at deep locations (>1?mm) within the mouse cerebral cortex. ? 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement 1. Introduction Multiphoton microscopy (MPM) is widely used in neuroscience to visualize neurons and vasculature in mouse models at depths up to approximately 1 mm in the cortical cortex [1?3]. Since MPM relies on the nonlinear